滨蒿内酯通过调节egfr/mapk通路诱导卵巢癌细胞凋亡，首发论文 -凯发网

scoparone induces ovarian cancer cell apoptosis induces apoptosis

• 1、college of pharmaceutical sciences, soochow university

abstract：objective: to investigate the effects of scoparone on the functions of ovarian cancer cells. methods: mtt and colony formation assays were used to examine the effects of scoparone on the proliferation ability of ovarian cancer cell lines. scratch wound healing assay and transwell invasion assay were performed to detect the effects of scoparone on the migration and invasion abilities of ovarian cancer cell lines. western blot (wb) and flow cytometry were employed to evaluate the apoptosis level of ovarian cancer cells after scoparone treatment. the swiss online website was used to predict the potential binding proteins of scoparone, and gene ontology (go) and kyoto encyclopedia of genes and genomes (kegg) analyses were conducted to enrich protein pathways. the predicted results of scoparone-binding proteins were verified by wb technology. results: mtt and colony formation assays revealed that scoparone effectively inhibited the proliferation ability of ovarian cancer cells. scratch wound healing and invasion assays showed that scoparone significantly suppressed the migration and invasion abilities of ovarian cancer. wb and flow cytometry results indicated that scoparone effectively promoted the apoptosis level of ovarian cancer cells. the swiss online analysis website revealed that the potential binding proteins of scoparone overlapped with the apoptosis signaling pathway. after wb verification, scoparone regulated the egfr/mapk signaling pathway. conclusion: scoparone significantly inhibits the proliferation, migration, and invasion levels of ovarian cancer cells and promotes apoptosis through the egfr/mapk signaling pathway.

keywords： tumor pharmacology scoparone ovarian cancer apoptosis