提示
氧化应激条件下黑磷纳米片诱导的毒性及其潜在机理研究
首发时间：2024-06-19
摘要：黑磷（black phosphorus, bp）纳米材料因自身独特的理化性质使其具有广泛的应用前景，因此bp纳米材料的生物安全性近年来也备受关注。然而，目前关于bp纳米材料的生物安全性研究多集中于正常生理环境，在病理状态下的研究则相对匮乏。目的：以体外培养的细胞和模式生物秀丽隐杆线虫为研究对象，研究氧化应激条件下bp纳米片对细胞和线虫的毒性效应及潜在机制。结果：研究表明正常条件下bp纳米片对细胞及线虫并未产生明显的毒性效应；然而，当细胞和线虫处于氧化应激状态下，bp纳米片显示出显著的细胞毒性和线虫毒性。进一步机制研究表明，bp纳米片与诱导氧化应激的过氧化氢发生反应产生大量羟基自由基，导致线粒体结构破坏并使细胞色素c从线粒体泄漏至细胞质。与此同时，bp纳米片在过氧化氢作用下发生降解，使其易于进入细胞并与细胞色素c作用，进而破坏细胞内抗氧化防御体系，最终导致生物毒性效应的产生。结论：本研究揭示了bp纳米片在氧化应激条件下诱导体外和体内毒性的潜在机制，为bp纳米材料在生物医学领域的应用提供了重要的安全性依据。
关键词：
for information in english, please click here
unraveling the stress-induced toxicity of black phosphorus nanosheets and the underlying mechanism
abstract：the unique physicochemical properties of black phosphorus (bp) nanomaterials make them extremely versatile, and growing concern has emerged regarding their biocompatibility. however, the toxicity of bp nanomaterials has mostly been studied under normal physiological conditions, while the studies on their toxicity under pathological conditions are rather limited. purpose: to investigate the toxic profile of bp nanosheets under oxidative stress conditions in both living cells and a simple animal model, caenorhabditis elegans. results: under normal conditions, bp nanosheets do not exert any adverse effects on cells and worms. however, bp nanosheets exhibit significant toxicity when cells and worms are under oxidative stress. further mechanistic studies indicate that hydroxyl radical overproduction is induced by the reaction between bp nanosheets and hydrogen peroxide, which may disrupt mitochondrial integrity and promote the leakage of cytochrome c from mitochondria into cytoplasm. meanwhile, bp nanosheets were degraded under oxidative stress conditions, providing opportunities for bp nanosheets to interact with cytochrome c, thereby disrupting the cellular antioxidant defense system, ultimately producing toxicity. conclusion: this researchuncovers the potential mechanism of bp nanosheets with oxidative stress-induced toxicity both in vitro and in vivo, providingimportant safety evidence for the application of bp nanomaterials in biomedical field.
keywords：
基金：
论文图表：
引用
导出参考文献
no.****
动态公开评议
共计0人参与
勘误表
氧化应激条件下黑磷纳米片诱导的毒性及其潜在机理研究
评论
全部评论