simultaneous determination of purine bases, ribonucleosides and ribonucleotides by capillary electrophoresis-凯发网

simultaneous determination of purine bases, ribonucleosides and ribonucleotides was achieved by coupling capillary electrophoresis (ce) with wall-jet amperometric detection. a 200um diameter copper disk electrode was applied at working potential, 0.65 v vs. saturated calomel electrode. the current response of high sensitivity and stability was obtained in strong basic solutions which were suitable for satisfactory ce separations. the calibration curve was linear over 2-3 orders of magnitude and the limits of detection for adenine, guanine, xanthine, uric acid, adenosine, guanosine, adenosine-5-monophosphate and guanosine-5'-monophosphate were below 9 fmol (s/n=3). the use of this method for the separation and detection of compounds present in human plasma samples was reported.