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期刊论文
preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from vibro sp.510
carbohydrate research 339(2004)1475-1481，-0001，（）：
alginate that was purified from the fermentation solution of marine bacteria vibro sp.510 under specific reaction conditions was hydrolyzed by alginate lyase. seven oligosaccharides, including di-, tri-and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (lp-gpc) and semipreparative strong-anion exchange (sax) fast-protein liquid chromatography (fplc). the oligosaccharide structures were elucidated based on esims and 2d nmr spectral analysis. the hydrolytic specificity of this alginate lyase to alginate is discussed.
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