preparation and characterization of oligomannuronates from alginate degraded by hydrogen peroxide，成果详细信息-凯发网

macromolecular alginate extracted from brown seaweeds was treated with acid to generate mannuronate blocks. the resulting polymannuronates were degraded by hydrogen peroxide with a procedure optimized for obtaining high recovery of low molecular weight saccharides. the resulted mixture of different sized oligomannuronates was separated by gel chromatography on a bio-gel p-4 column and mono q column. the purified oligomers were characterized by anion-exchange hplc as well as spectrum analysis. this report provides a general procedure for generation and purification of oligomannuronates with in the size of 2-7 sugar units from alginate.