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since its discovery, anthraquinone has become very valuable as a lead compound in the development of anti-cancer drugs. previously, we designed and synthesized a new type of amide anthraquinone derivative (1-nitro-2-acylanthraquinone glycine, c10) with good activity against colon cancer. however, its effect and the underlying mechanism are unclear. in this study, c10 significantly inhibited the proliferation of hct116 and ht29 colon cancer cells by blocking the cell cycle at the g2/m phase. c10 also plays a role in cell cycle arrest by reducing the protein and gene expression levels of cyclin b1 and its downstream signaling molecule cyclin-dependent kinase (cdk1). in addition, molecular docking studies showed that c10 has high affinity for jak2, the first target in the cell cycle-related jak2/stat3 signaling pathway. furthermore, c10 downregulated the expression of jak2/stat3 signaling pathway-related signaling molecules proteins and genes, and up-regulated the expression of pias-3, the upstream signaling molecule of stat3, thereby down-regulating stat3 phosphorylation.